Plant Tissue Culture Media
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Recent Advances in Plant in vitro Culture. Optimal growth and morphogenesis of tissues may vary for different plants according to their nutritional requirements. Moreover, tissues from different parts of plants may also have different requirements for satisfactory growth [ 1 ]. Tissue culture media were first developed from nutrient solutions used for culturing whole plants e. Plant tissue culture media should generally contain some or all of the following components: macronutrients, micronutrients, vitamins, amino acids or nitrogen supplements, source s of carbon, undefined organic supplements, growth regulators and solidifying agents. According to the International Association for Plant Physiology, the elements in concentrations greater than 0. It should be considered that the optimum concentration of each nutrient for achieving maximum growth rates varies among species.
Abstract A synthetic, chemically defined medium was developed for the initiation and growth of cell and tissue cultures of red clover Trifolium pratense L.
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